The Assessment of Neuronal Density of CA1 and CA2 Regions in Hippocampus of Newborn Rats Born from Mothers Fed by Cantharellus cibarius Hidroalcoholic Extract

One of the characteristics of human is the ability to change behavior based on previous experience and learning is the gaining the information that makes it possible. Memory is somewhere the preserve and storing this information. The hippocampus is one of centers in the brain responsible for learning and this center is a part of limbic system where new neurons are generated through neurogenesis. The aim of this study was to assess the changes in neuronal density in CA1 and CA2 regions in hippocampus of rats born from mothers fed by Cantharellus cibarius extract. In this experimental study, 24 female Wistar rats weighing 200 250 g were randomly divided to randomize controlled and 1, 2 and 3 treated groups with Cantharellus cibarius hydroalcoholic extract with doses of 100, 250 and 500. Treatment was performed through gavage method 7 days before mating. After delivery, one newborn rat of each mother was accidentally selected and anesthetized with ether, and after removing the skull, tissue processing was per182 Zahra Esmaili and Maryam Tehranipour formed in order to study the neuronal density in CA1 and CA2 areas by stereology and dissector methods, and all findings were compared by using ANOVA and t-test at significance level, (p<0.05).In control and 1, 2 and 3 experimental groups, the mean of neuronal density of CA1 was 2423 ±1.59, 3425±183, 4003 ± 101 and 4822 ±19.5, respectively, in CA2 area it was 2066±390, 3568±150, 3993 ±65.05 and 4629 ±64.7. Statistical analysis showed significant increase in neuronal density in (CA1, CA2) areas in experimental group (doses of 100, 250 and 500 mg / kg hydroalcoholic extract) compared to the control group, (p<0.05). It is possible that the Cantharellus cibarius extract increases the neurogenesis by excess neuronal density due to minerals such as magnesium, calcium, zinc, vitamin D and vitamin E.